High-dimensional spatial biology approach to study tumor budding Group Zlobec In our recently funded Innosuisse project together with Lunaphore technologies, we are establishing a high-dimensional protein expression panel to investigate the nature of tumor buds and their microenvironments under native and treatment scenarios. We investigate the “active” state of tumor buds and their relationship to Epithelial-Mesenchymal Transition (EMT). Most importantly, the clinical relevance of different budding phenotypes, stromal changes and immune cell contexture under different conditions are interrogated by utilizing our well-documented patient collectives and ngTMA®. Data analysis is critical and we aim to develop streamlined pipelines to evaluate these multiplexed fluorescent images using in-house deep learning algorithms and commercially available and open-source software. Tumor microenvironment in colorectal cancer at 20x magnification: a, Panck (red) and Vimentin (green); b, CD20 (pink) and CD3 (yellow); c, E-cadherin (green) and CDX2 (red).