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Research: Apoptosis - Microbeam Radiation Therapy - Osteo-articular Research - Mucosal Immunology - Leukocyte & Tumor Cell Migration - Peptide Receptors - Vascular Diseases - Virosomes - Publications |
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Mucosal Immunology (PI:Christoph Müller) |
Research Project: Analysis of the T cell receptor repertoire of distinct intestinal T cell subsets: influence of intestinal location on the TCR repertoire. |
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Silvia Rihs, Erik Vassella |
| By using a RT-PCR based amplification of TCR Vb-Cb, followed by a Jb primer-driven run-off reaction, the length of the T cell receptor's (TCR's) third complementarity determining region (CDR3) is determined. Hence, the entire polyclonal pool of TCRab T cells can be subdivided into 288 different groups (“spectratyping”; “immunoscope”). Such a fine-tuned analysis allows to follow clonal expansion, and regression, of given T cell clones, e.g. in infectious diseases, or in immunopathological disorders. |
| Spectratyping for all mouse TCR Vb families was established using a ABI Prism® 3100 Avant Genetic Analyzer to follow the TCR repertoire of distinct T cell subsets in defined segments of the small and large intestine of the mouse. | |
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T cell receptor - spectratyping Jb primer driven run off reactions from a TCR Vb13 - Jb product. Amplification fo Vb - Jb fragments using distinctly labeled Jb primers allows the simultaneous detection of TCRb spectratypes with different Jb's.
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Mucosal immunity: Immune reaction in intestinal mucosa - TNF in colitis - TCR repertoire - Intestinal macrophages - Transmembrane TNF |
| Page last updated: 23/08/06 |
Institute of Pathology |
Contact: contact@patho.unibe.ch |